Titusville is a city in and the county seat of Brevard County, Florida, United States. The city's population was 43,761 as of the 2010 United States Census. Titusville is on the Indian River, west of Merritt Island and the Kennedy Space Center, and south-southwest of the Canaveral National Seashore. It is a principal city of the Palm Bay–Melbourne–Titusville Metropolitan Statistical Area.